No, the Riddler, Joker and Penguin were all brought back by Julie before '66.

In fact, it's believed that the Riddler's return was one of the things that inspired Dozier to green-light the Batman TV show.


.

"As for contentment, that is a myth.  Give a man everything he wishes and he will be unhappy, because he didn't wish for more; give him more, and he will die of his worries.  Only a turtle, asleep on a sunny log, knows contentment!"
-Merlin to Prince Valiant

            Hal Foster 3-7-43